J Mingo, A Erramuzpe, S Luna, I Diez, JM Cortes and R Pulido. One-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution Collections. PLoS One 11: e0160972, 2016 [pdf] Site-directed mutagenesis (SDM) is a powerful tool to create defined collections of protein variants for experimental and clinical purposes, but effectiveness is compromised when a large number of mutations is required. We present here a one-tube-only standardized SDM […]
Daily Archives: August 24, 2016
Lopez JI and Cortes JM. A multi-site cutting device implements efficiently the divide-and-conquer strategy in tumor sampling. F1000Research 5:1587, 2016 [pdf] We recently showed that in order to detect intra-tumor heterogeneity a Divide-and-Conquer (DAC) strategy of tumor sampling outperforms current routine protocols. This paper is a continuation of this work, but here we focus on DAC implementation in the Pathology Laboratory. In particular, we describe a new simple method that […]
Guarch R, Cortes JM, Lawrie CH and Lopez JI. Multi-site tumor sampling (MSTS) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (CCRCC). F1000Research 5: 2020, 2016 [pdf] Current standard-of-care tumor sampling protocols for CCRCC (and other cancers) are not efficient at detecting intratumoural heterogeneity (ITH). We have demonstrated that an alternative protocol, multi-site tumor in silico sampling (MSTS) based upon the divide and […]